Next Generation Sequencing: Approach for Assessment and enrichment of raw data
Next-generation sequencing (NGS) is a non-sanger technique based on high-throughput capacity for determining the exact order of nucleotide in the given DNA & RNA molecule. Modern high-throughput sequencer’s produce millions of sequences read in a single run. The quality of these data are not perfect because they contains artefacts like poor quality reads, adapter contamination, wrong base calls and INDELs due to instrument failure or chemical process error. In this study, a procedure has been designed for the refinement and improvement of quality of NGS data for further biological processes.
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