Protein Separation Using Fly-ash Microfiltration Ceramic Membrane

  • Vandana Gupta Department of Chemical Engineering, National Institute of Technology Raipur
  • Anandkumar J Department of Chemical Engineering, NIT Raipur
Keywords: BSA separation, ceramic membrane, fly-ash, microfiltration.

Abstract

In this study, separation of protein (bovine serum albumin (BSA)) was carried out by ceramic microfiltration membranes. Ceramic membranes were fabricated by using fly-ash with different proportion (2-8 wt%) of fuller clay and fraction (20 wt%) of inorganic additives. Synthesized ceramic membranes were characterized using scanning electron microscope, X-ray diffraction analysis, mechanical-chemical stability, porosity and pure water flux. It was observed that the mechanical and chemical stability of ceramic membrane increases with increase in fuller clay’s content. Ceramic membrane with 8% fuller clay (C4) exhibited maximum flexural strength of 20 MPa. C4 membrane also shows least porosity of 29.9%, permeability of 0.397 L m-2h-1kPa-1, 20.15% water uptake capacity and 0.428 μm average pore radius. The BSA rejection efficiency of C4 membrane was studied for different operating parameter such as feed concentration (200-1000 mg/L), feed pH (2-10) and applied pressure (68-482 kPa). Maximum BSA rejection (82%) and flux (81 L m-2 h-1) has been observed at optimized condition (208 kPa, natural pH and 200 mg/L concentration). The results obtained in this work indicate that synthesized membrane could be used as proficient microfiltration membrane for protein rejection applications.

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Published
2018-09-01
How to Cite
Gupta, V., & J, A. (2018). Protein Separation Using Fly-ash Microfiltration Ceramic Membrane. CSVTU International Journal of Biotechnology, Bioinformatics and Biomedical, 3(2), 17-25. https://doi.org/https://doi.org/10.30732/ijbbb.20180302002
Section
Articles