Protein Separation Using Fly-ash Microfiltration Ceramic Membrane
In this study, separation of protein (bovine serum albumin (BSA)) was carried out by ceramic microfiltration membranes. Ceramic membranes were fabricated by using fly-ash with different proportion (2-8 wt%) of fuller clay and fraction (20 wt%) of inorganic additives. Synthesized ceramic membranes were characterized using scanning electron microscope, X-ray diffraction analysis, mechanical-chemical stability, porosity and pure water flux. It was observed that the mechanical and chemical stability of ceramic membrane increases with increase in fuller clay’s content. Ceramic membrane with 8% fuller clay (C4) exhibited maximum flexural strength of 20 MPa. C4 membrane also shows least porosity of 29.9%, permeability of 0.397 L m-2h-1kPa-1, 20.15% water uptake capacity and 0.428 μm average pore radius. The BSA rejection efficiency of C4 membrane was studied for different operating parameter such as feed concentration (200-1000 mg/L), feed pH (2-10) and applied pressure (68-482 kPa). Maximum BSA rejection (82%) and flux (81 L m-2 h-1) has been observed at optimized condition (208 kPa, natural pH and 200 mg/L concentration). The results obtained in this work indicate that synthesized membrane could be used as proficient microfiltration membrane for protein rejection applications.
 Fluegel, S.M., 2013. Dairy bioactives: BSA. https://mooscience.com/Bovine-Serum-Albumin.html (accessed 20 march 20018).
 https://rockland-inc.com/bovine-serum-albumin.aspx. (accessed 20 march 20018).
 Pereira, M.M., Pedro, S.N., Quental, M.V., Lima, A.S., Coutinho, J.A.P., Freire M.G., 2015. Enhanced extraction of bovine serum albumin with aqueous biphasic systems of phosphonium- and ammonium-based ionic liquids. Journal of Biotechnology, 206, 17–25.
 Taha, M., Quentala, M.V., Correia, I., Freire, M.G., Coutinho, J.A.P., 2015. Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good’s buffers ionic liquids. Process Biochemistry, 50, 1158–1166.
 Hebbar, H.U., Raghavarao, K.S.M.S., 2007. Extraction of bovine serum albumin using nanoparticulate reverse micelles. Process Biochemistry, 42, 1602–1608.
 Chen, L., Dong, J., Guo, X., 2017. Extraction of bovine serum albumin with reverse micelles from glucosylammonium and lactosylammonium surfactants. Process Biochemistry, 60, 108–114.
 Pawar, S.S., Regupathi, I., Prasanna, B.D., 2017. Reverse micellar partitioning of Bovine Serum Albumin with novel system. Resource-Efficient Technologies, 3, 491–494.
 Pramila, J., Melbiah, J.S.B., Rana, B., Gandhi, N.N., Nagendran, A., Mohan, D., 2018. Permeation characteristics of tailored poly (m-phenylene isophthalamide) ultrafiltration membranes and probing its efficacy on bovine serum albumin separation. Polymer Testing, 67, 218–227.
 Saraswathi, M.S.A., Mahalakshmi, S., Vetrivel, S., Divya, K., Rana, D., Nagendran A., 2018. Separation of bovine serum albumin and humic acid contaminants from aqueous stream using tailored poly (amide imide) ultrafiltration membranes. Journal of Environmental Chemical Engineering, 6, 1912–1917.
 Gebru, K.B., Das, C., 2017. Removal of bovine serum albumin from wastewater using fouling resistant ultrafiltration membranes based on the blends of cellulose acetate, and PVP-TiO2 nanoparticles. Journal of Environmental Management, 200, 283-294.
 Yang, L., Haitao, H., Pengfei, H., Jiyou, G., 2017. Exploration of zwitterionic cellulose acetate antifouling ultrafiltration membrane for bovine serum albumin (BSA) separation. Carbohydrate Polymers. 165, 266-275 (http://dx.doi.org/10.1016/j.carbpol.2017.02.052)
 Valiño, V., Román, M.F.S., Ibañez, R., Ortiz, I., 2014. Improved separation of bovine serum albumin and lactoferrin mixtures using charged ultrafiltration membranes. Separation and Purification Technology, 125, 163–169.
 Teepakorn, C., Fiaty, K., Charcosset, C., 2015. Optimization of lactoferrin and bovine serum albumin separation using ion-exchange membrane chromatography. Separation and Purification Technology, 151, 292–302.
 Fakhfakh, S., Baklouti, S. & Bouaziz, J., 2010. Preparation ,characterization and application in BSA solution of silica ceramic membranes. Desalination, 262, 188-195.
 Jedidi, I., Sai, S., Khmakem, S., Elloumi-ammar, N., Fourati, A., Charf, A., 2009. New ceramic microfiltration membranes from mineral coal fly ash. Arabian Journal of Chemistry, 2, 31-39.
 Vasanth, D., Pugazhenthi, G., Uppaluri, R., 2013. Cross flow microfiltration of oil-in-water emulsion using low cost ceramic membranes. Desalination, 320, 86-95.
 Jana, S., Purkait, M.K., Mohanty, K., 2011. Clay supported polyvinyl acetate coated composite membrane by modified dip coating method: Application for the purification of lysozyme from chicken egg white. Journal of Membrane Science, 382, 243–251.
 Tanyolac, D., Sonmezisik, H., Ozdural, A.R., 2005. A low cost porous polyvinylbutyral membrane for BSA adsorption. Biochemical Engineering Journal, 22, 221-228.
 Mittal, P., Jana, S., Mohanty, K., 2011. Synthesis of low-cost hydrophilic ceramic-polymeric composite membrane for treatment of oily wastewater. Desalination, 282, 54-62.
 Hashino, M., Hirami, K., Ishigami, T., Ohmukai, Y., Maruyama, T., Kubota, N., Matsyama, H., 2011. Journal of Membrane Science, 384, 157-165.
 Jedidi, I., Khemakhem S., Saïdi S., Larbot A., Elloumi-Ammar N., Fourati A., Charfi, A., Salaha, A.B., Amar, R.B., 2011. Preparation of a new ceramic microfiltration membrane from mineral coal fly ash: Application to the treatment of the textile dying effluents. Powder Technology, 208, 427–432.
 Vasanth, D., Pugazhenthi, G., Uppaluri, G., 2011. Fabrication and properties of low cost ceramic microfiltration membranes for separation of oil and bacteria from its solution. Journal of Membrane Science, 379, 154–163.
 Porubská, M., Szöllos, O., Kónová, A., 2012. FTIR spectroscopy study of polyamide-6 irradiated by electron and proton beams. Polymer Degradation and Stability, 97, 523-531.
 Sinha, M.K., Purkait, M.K., 2013. Increase in hydrophilicity of polysulfone membrane using polyethylene glycol methyl ether. Journal of Membrane Science, 437, 7-16.
 Aravind, K.U., Mathew, J., Aravindkumar, C.T., 2007. Transport studies of BSA, lysozyme and ovalbumin through chitosan/polystyrene sulfone multilayer membrane. Journal of Membrane Science, 299, 146-155.
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