Assessment of phylogenetic relationship of Jatropha based on nuclear ITS regions (ITS/5.8S) and chloroplast (cp) matK gene

  • Adreeja Basu Department of Biological Sciences, St. John’s University, Queens, New York 11439
  • Shree Rajkumar Saha Department of Mathematics, The City College of New York, New York 10031
  • Lingaraj Sahoo Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India
Keywords: ITS spacers, maturaseK, phylogenetics, Jatropha

Abstract

The phylogenetic relationships between the toxic and the non-toxic variety of Jatropha curcas and its sister species Jatropha podagrica and Jatropha gossypifolia from North East India were assessed using the internal transcribed spacer region of nuclear ribosomal DNA (nrDNA ITS) and the maturaseK gene (matK) of chloroplast. For both the marker system, the maximum genetic distance was maintained between J. podagrica and J. curcas. The % (G + C) content of the matK region was markedly lower than that of the ITS region.  ITS biomarkers were able to detect 111 variable sites while only 4 substitutions were detected in highly conserved matK region. The obtained results delineated that, in comparison to matK, ITS sequencing can provide valuable information during interpopulation analysis of Jatropha species.

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Published
2019-05-07
How to Cite
Basu, A., Saha, S., & Sahoo, L. (2019). Assessment of phylogenetic relationship of Jatropha based on nuclear ITS regions (ITS/5.8S) and chloroplast (cp) matK gene. CSVTU International Journal of Biotechnology, Bioinformatics and Biomedical, 4(1), 13-20. Retrieved from http://csvtujournal.in/index.php/ijbbb/article/view/38
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Articles