Assessment of phylogenetic relationship of Jatropha based on nuclear ITS regions (ITS/5.8S) and chloroplast (cp) matK gene
The phylogenetic relationships between the toxic and the non-toxic variety of Jatropha curcas and its sister species Jatropha podagrica and Jatropha gossypifolia from North East India were assessed using the internal transcribed spacer region of nuclear ribosomal DNA (nrDNA ITS) and the maturaseK gene (matK) of chloroplast. For both the marker system, the maximum genetic distance was maintained between J. podagrica and J. curcas. The % (G + C) content of the matK region was markedly lower than that of the ITS region. ITS biomarkers were able to detect 111 variable sites while only 4 substitutions were detected in highly conserved matK region. The obtained results delineated that, in comparison to matK, ITS sequencing can provide valuable information during interpopulation analysis of Jatropha species.
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