http://csvtujournal.in/index.php/ijbbb/issue/feed CSVTU International Journal of Biotechnology, Bioinformatics and Biomedical 2018-05-21T12:36:58+0530 Dr. Biju P. Sahariah csvtuijbbb@csvtu.ac.in Open Journal Systems <p>The International journal of Biotechnology, Bioinformatics and Biomedical encompasses multidisciplinary fields of Biotechnology, Bioinformatics and Biomedical. It endeavours pure and applied knowledges through advanced methodologies, technologies and or any new area which has potential in these areas and application of scientific inventions for benefit of the society. We invite scholars, academicians and all scientists’ society to share their valuable research in the form of review, research article, short communications through this platform for the better development and betterment of one and only earth. The aim of <strong>International Journal of Biotechnology, Bioinformatics, and Biomedical (IJBBB)</strong> is to propagate the knowledge and establish the communication amongst the researchers, academicians, industry personnel and policy makers.&nbsp;</p> http://csvtujournal.in/index.php/ijbbb/article/view/23 Production of Titanium Dioxide Nanoparticle Using Baker’s Yeast 2018-05-09T10:25:19+0530 Shevi Chaturvedi shevichaturvedi@gmail.com Abhishek Kumar abhishekchaudhary964@gmail.com <p>Micro-organisms like bacteria, cynobacteria, actinomycetes and fungi are employed to synthesise nanoparticles. The yeast strain possess more benefits over bacteria because they are easy to control in laboratory, synthesise numerous enzymes and rapid growth with the use of simple nutrients. In this study Luria- Bertani (LB) media was used for the culture of Baker’s yeast to prepare titanium dioxide nanoparticles. Titanium dioxide (i.e. Ti0<sub>2</sub>) is also known as Titania. Titanium dioxide has many functions in various industries, it is widely used in the sunscreens to block the UV-rays and many more. Yeast cells were allowed to grow as suspension culture and this culture was treated as source culture. A small portion of this culture (25 ml) was filtered and diluted four times by adding 30% ethanol. TiO<sub>2</sub> solution was added to the culture solution and the culture was allowed to incubate. The nanoparticles of titanium were filtered under the laminar flow by using Whatman filter paper.</p> 2018-04-30T00:00:00+0530 ##submission.copyrightStatement## http://csvtujournal.in/index.php/ijbbb/article/view/25 Production, purification and characterization of Inulinase from fungus of dahlia rhizosphere 2018-05-21T12:36:58+0530 Shaloo Verma frndzsverma123@gmail.com Ratna Prabha ratnasinghbiotech30@gmail.com Anand Mohan anandmohan77@gmail.com <p>Inulinase and its studies are of immense importance in the present scenario. The use of microbial inulinases is suggested as a very potential &nbsp;approach, however, for obtaining inulinase in sufficient quantity from microbes, particularly fungii, optimization of the growth parameters&nbsp; is essential. The current study is focused over extraction of inulinase from fungal strains isolated from rhizospheric soil of dahlia plant and optimization of growth parameters. In a total of ten isolates, two organisms <em>Fusarium oxysporum</em> and <em>Aspergillus</em> sp. were identified as efficient inulinase producers. The optimum incubation time, temperature and pH of these organisms were found to be 40 min, 65°C at 6.5 pH and 30 min, 50°C at 6.5 pH respectively. Inulin was identified as appropriate carbon source for these microbes. Thin layer chromatography validated the presence of hydrolysis product via the &nbsp;action of inulinase on inulin into fructose unit. The highest enzyme activity and Specific activity was obtained from <em>Aspergillus</em> spp. for 20% fractionate was 1.81U/ml and 2.288U/mg, for 40% fractionate was 0.735U/ml and 1.047U/mg and for 60% fractionate was 3.74U/ml and 12.47U/mg respectively at the optimized condition. DEAE Sephadex A 50 Ion exchange column chromatography show that the fifth fraction of 20% fractionate had shown the maximum enzyme activity.&nbsp; The molecular weight of this enzyme was estimated at 66±2kDa and 43±2kDa using SDS-PAGE after ammonium sulphate precipitation, dialysis and chromatography analysis which showed exo-inulinase type.</p> 2018-05-04T00:00:00+0530 ##submission.copyrightStatement## http://csvtujournal.in/index.php/ijbbb/article/view/26 Bioreactors for efficient treatment of phenol and nitrogen from wastewater 2018-05-09T10:30:17+0530 Biju Prava Sahariah biju.sahariah@gmail.com <p>Bioreactors are operated in aerobic, anaerobic and anoxic environment either as single unit or in series of sequence for treatment of various types of wastewater for desirable outcome. The working force microbes grow in suspension in suspended growth reactor configuration or may be grown in attachment of some support media (mostly referred as bed) in attached growth reactor configuration. Therefore, in suspended system, microbes are kept suspended in the liquor whereas in attached growth system, some carrier material is provided in the reactors for use as a bed for microbial growth. Both suspended growth and attached growth bioreactors are used for removal of complex wastewater containing inorganics, organics and various metals. The review article possesses an insight of comparison of various reactors used for treatment of complex wastewater with phenol and ammonia component.</p> 2018-05-07T11:07:49+0530 ##submission.copyrightStatement##