Production of Titanium Dioxide Nanoparticle Using Baker’s Yeast

  • Shevi Chaturvedi ITM University, Gwalior
  • Abhishek Kumar Department of Biotechnology, ITM University Gwalior, Madhya Pradesh, India
Keywords: Nanoparticles;, Eco- friendly, Green synthesis, UV-rays, Saccharomyces cerevisiae.


Micro-organisms like bacteria, cynobacteria, actinomycetes and fungi are employed to synthesise nanoparticles. The yeast strain possess more benefits over bacteria because they are easy to control in laboratory, synthesise numerous enzymes and rapid growth with the use of simple nutrients. In this study Luria- Bertani (LB) media was used for the culture of Baker’s yeast to prepare titanium dioxide nanoparticles. Titanium dioxide (i.e. Ti02) is also known as Titania. Titanium dioxide has many functions in various industries, it is widely used in the sunscreens to block the UV-rays and many more. Yeast cells were allowed to grow as suspension culture and this culture was treated as source culture. A small portion of this culture (25 ml) was filtered and diluted four times by adding 30% ethanol. TiO2 solution was added to the culture solution and the culture was allowed to incubate. The nanoparticles of titanium were filtered under the laminar flow by using Whatman filter paper.


1. Akhilesh Kushwaha, Vishal Kumar Singh, Juhi Bharteriya, Priya Singh and Khaleeja Yasmeen. Isolation and identification of E.coli bacteria for the synthesis of silver nanoparticles: Characterization of the particles and study of antibacterial activity. European Journal of Experimental Biology, 2015, 5(1): 65-70.
2. Parthiban S, Raja S, Mukhopadhya H K, Everyman’s Science, 2010, 2, 106.
3. Wang J and Wang Z 2007. Mater. Lett 61 4149.
4. Yokoyama K and Welchons D 2007. Nanotehnology 18, 105101.
5. Amin Boromand Moghaddam, Faridesh Namvar, Mona Moniri, Paridah Md. Tahir, Susan Azizi and Rosfarizan Mohamad. Nanoparticles Biosynthesised by fungi and yeast. A review of their preparation, properties and Medical Applications. Molecules 2015, 20, 16540- 16565.
6. Kumar, D; Karthik, L; Kumar, G; Roa, K.B. Biosynthesis of silver nanoparticles from Marine Yeast and their antimicrobial activity against multidrug resistant pathogens. Pharmacologyonline 2011, 3, 1100-1111.
7. Blackwell, M. The fungi: 1,2,3------5.1 million species? Am. J. Bot. 2011, 98, 426-438.
8. K. Prasad, Anal K. Jha, A.R. Kulkarni Lactobacillus assisted synthesis of titanium nanoparticles. Nanoscale Res Lett 2007, 2: 248-250.
9. Matej Skocaj, Metka Filipic, Jana petkovic and Sasa Novak. Titanium dioxide in our everyday life; is it safe? Radiol oncol 2011; 45 (4): 227-247.
10. Dave RH. Overview of pharmaceutical excipients used in tablets and capsuls. Drug Topic (online). Advanstar. 10/24/2008. topic/ article/article Detail. Jsp?id=561047. Accessed 08/19/2011.
11. Yang F, Hong, F, You W, Liu C, Wu C, et al. Mechanisum of nanoantase Tio & It, sub&It; sub& gt; on the nitrogen metabolism of growing spinach. Biol Trace Elem Res 2006; 110: 179- 90.
12. Gao F, Hong F, Liu C, Zheng L, Su M, Wu X, et al. Mechanism of nanoanatase Tio&It; sub&get; on promoting photosynthetic carbon reaction of spinach. Boil Trace Flem Res 2006; 111: 239-53.
13. Gao F, Liu C, Qu C, Zheng L, Yang F, Su M et al.Was improvement of spinch growth by nano- Tio &It; sub&It; 2&It;/ sub&It; treatment related to the changes of Rubisco activase? Biometals 2008; 212: 211-17.
14. Su Mg, Liu C, Qu Cx, Zheng L, Chen L, Huang H, et al. Nanoanatase relieves the inhibition of electron transport caused by linoenic acid in chloroplasts of spinach. Bio Trace Elem Res 2008; 122: 73-81.
15. Lu Cm, Zhang Cy, When Jq, Wu Gr: (in Chinese). Soyabean Sci 2002; 21: 168-71.
16. Anal K. Jha, K. Prashad and A.R, Kulkarni. Yeast Mediated Synthesis of Silver Nanoparticles. International Journal of Nanoscience and Nanotechnology 2008, 4 (1), 17-22.
How to Cite
Chaturvedi, S., & Kumar, A. (2018). Production of Titanium Dioxide Nanoparticle Using Baker’s Yeast. CSVTU International Journal of Biotechnology, Bioinformatics and Biomedical, 3(1), 01-05.