Effect of chemical pretreatments on de-oiled rice bran for fermentative biohydrogen production

Authors

  • Shabina Khan PTRSU
  • Veena Thakur
  • Afaque Quraishi School of Studies in Biotechnology, Pandit Ravishankar Shukla University Raipur, (C.G.), India
  • S. K. Jadhav School of Studies in Biotechnology, Pandit Ravishankar Shukla University Raipur, (C.G.), India

DOI:

https://doi.org/10.30732/ijbbb.20160101004

Keywords:

Biohydrogen, Clostridium acetobutylicum NCIM 2877 pretreatment, volumetric hydrogen, hydrolysate

Abstract

Increased demand of fossil fuels has lead to its shortfall and also for an urge for the search of alternative source of energy, which is non-polluting. Biohydrogen is an ideal fuel of future as it can be produced from different types of wastes, solids or liquids,  rich in organic matter and it does not produce any types of green house gases when combusted. Present work was carried out to enhance the biohydrogen production from de-oiled rice bran by pretreatment methods. Presence of high amount of carbohydrate (39% cellulose and 31% hemicellulose) and less lignin (4%) makes it an attractive feedstock for conversion into a variety of value-added products. De-oiled rice bran was taken as substrate and Clostridium acetobutylicum NCIM 2877 was used as seed culture for the fermentative biohydrogen production. Substrate was pretreated with different concentrations of acids and alkali. 1.5% H2SO4 pretreated de-oiled rice bran hydrosylate gave maximum of 97.5 ± 2.5 ml volumetric biohydrogen with conversion efficiency of 87.27 ± 0.84 % whereas maximum production of 130 ± 1.5 ml with a conversion efficiency of 65.66 ± 0.08 % was achieved at 0.25% of HCL. Among alkali, 0.1% NaOH pretreated de-oiled rice bran hydrosylate gave 99.0 ± 3.0 ml production with conversion efficiency of 83.36 ± 0.91 % and  0.75 % Ca(OH )2 pretreated de-oiled rice bran hydrosylate gave maximum production of  73.78 ± 7.4 ml. Therefore, by pretreatment of various complex biomasses, production of biohydrogen can be enhanced

References

Balat M. (2009) Bioethanol as a vehicular fuel: a critical review. Energy Sources Part A; 31:1242-55
2. Demirbas M.F. (2009) Bio-oils from corn stover via supercritical water liquefaction. Energy Educ Sci Technol Part A; 23: 97-104.
3. Kim, S.H., Han, S.K., and Shim, H.S. (2004) Feasibility of Biohydrogen production by anaerobic co- digestion of food waste and sewage sludge Int J Hydrogen Energy, 29:1607–16.
4. Lui, Y., YU, P., Song, X. and Qu, Y. (2008) Hydrogen production from cellulose by co culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharotylicuim Gd17 Int. J. Hydrogen Energy. 33. 2927-2933.
5. Midilli A., Ay M., Dincer I., Rosan M.A. (2005) On hydrogen and hydrogen energy strategies I: current status and needs. Renewable and sustainable energy reviews, 9, 255-60.
6. Ozmihci S and Kargi F. (2011) Dark fermentative bio-hydrogen production from waste wheat starch using co-culture with periodic feeding: effects of substrate loading rate. Int. J of Hydrogen Energy; 36:7089–7093.
7. Sen, M. and Banerjee, R. (2008) Comparison of biohydrogen production processes. Int. Journal of Hydrogen Energy, 33:279 – 286.
8. Saha, B.C. Iten, L.B., Cotta, M.A. and Wu, Y. U. (2005) Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol,” Biotechnology Progress, 21(3), 816–822.
9. Thakur Veena., Tiwari K.L., Quraishi A. and Jadhav S.K. (2012) Biohydrogen production from rice mill effluent. Journal of Applied Science in Environmental Sanitation, 7(4) 237-240.
10. Wang, J., Wan, W., (2009) Factors influencing fermentative hydrogen production: a review. Int. J. Hydrogen Energy 34, 799–811.
11. Wang, A., Ren, N., Shi, Y., Lee, D.J. (2008) Bioaugmented hydrogen production from microcrystalline cellulose using co-culture – Clostridium acetobutyricum X9 and Ethanoligenens harbinense B49. Int J Hydrogen Energy 33:912–917.
12. Zhao, Y. Wang, J. Y. Zhu, A. Ragauskas, and Y. Deng, (2008) “Enhanced enzymatic hydrolysis of spruce by alkaline pretreatment at low temperature,” Biotechnology and Bioengineering, 99 (6):1320–1328.

Downloads

Published

2017-05-06

How to Cite

Khan, S., Thakur, V., Quraishi, A., & Jadhav, S. K. (2017). Effect of chemical pretreatments on de-oiled rice bran for fermentative biohydrogen production. CSVTU International Journal of Biotechnology, Bioinformatics and Biomedical, 1(1), 20–24. https://doi.org/10.30732/ijbbb.20160101004

Issue

Vol. 1 No. 1 (2016): Peer Review

Section

Articles

License

The Copyright Notice will appear in About the Journal. It should describe for readers and authors whether the copyright holder is the author, journal, or a third party. It should include additional licensing agreements (e.g. CREATIVE COMMONS licenses) that grant rights to readers (see EXAMPLES), and it should provide the means for securing permissions, if necessary, for the use of the journal's content