Production and in-situ localization of ROS in Pennisetum typhoideum indulged with heavy metal stress

Authors

  • Gunjan Rughani ptrsu
  • Jipsi Chandra School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India
  • Vibhuti Chandrakar School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India
  • S. Keshavkant School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India

DOI:

https://doi.org/10.30732/ijbbb.20160101002

Keywords:

Reactive oxygen species, Radicles

Abstract

Reactive oxygen species (ROS) play key roles in seed germination and seedling establishment and was shown to perform both beneficial and damaging roles, depending upon concentration. At low level, it has been shown to boost plant growth, while high existence is injurious. In view to investigate the accumulation and consequent functions of ROS during heavy metal (Nickel, Ni) stress on Pennisetum typhoideum, its seeds were subjected to Ni stress (NiCl2 concentration: 0 ppm, 50 ppm, 100 ppm, 150 ppm, 250 ppm and 350 ppm) their radicles were evaluated for change in physiological trait (root length). Contents of both superoxide (O2•−) and hydrogen peroxide (H2O2) were determined. Moreover, radicles were stained with nitro blue tetrazolium and 3, 3-diaminobenzidine to localize the precise site(s) of O2•− and H2O2 production. Accumulated data revealed, significant fall in length of Pennisetum typhoideum radicles in response to Ni stress. Further, under Ni treatment, remarkable rise in contents and spread in the sites of both ROS (O2•− and H2O2) production was observed.

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Published

2017-05-06

How to Cite

Rughani, G., Chandra, J., Chandrakar, V., & Keshavkant, S. (2017). Production and in-situ localization of ROS in Pennisetum typhoideum indulged with heavy metal stress. CSVTU International Journal of Biotechnology, Bioinformatics and Biomedical, 1(1), 8–13. https://doi.org/10.30732/ijbbb.20160101002

Issue

Vol. 1 No. 1 (2016): Peer Review

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Articles

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