Protein Separation Using Fly-ash Microfiltration Ceramic Membrane

Authors

  • Vandana Gupta Department of Chemical Engineering, National Institute of Technology Raipur
  • Anandkumar J Department of Chemical Engineering, NIT Raipur

DOI:

https://doi.org/10.30732/ijbbb.20180302002

Keywords:

BSA separation, ceramic membrane, fly-ash, microfiltration.

Abstract

In this study, separation of protein (bovine serum albumin (BSA)) was carried out by ceramic microfiltration membranes. Ceramic membranes were fabricated by using fly-ash with different proportion (2-8 wt%) of fuller clay and fraction (20 wt%) of inorganic additives. Synthesized ceramic membranes were characterized using scanning electron microscope, X-ray diffraction analysis, mechanical-chemical stability, porosity and pure water flux. It was observed that the mechanical and chemical stability of ceramic membrane increases with increase in fuller clay’s content. Ceramic membrane with 8% fuller clay (C4) exhibited maximum flexural strength of 20 MPa. C4 membrane also shows least porosity of 29.9%, permeability of 0.397 L m-2h-1kPa-1, 20.15% water uptake capacity and 0.428 μm average pore radius. The BSA rejection efficiency of C4 membrane was studied for different operating parameter such as feed concentration (200-1000 mg/L), feed pH (2-10) and applied pressure (68-482 kPa). Maximum BSA rejection (82%) and flux (81 L m-2 h-1) has been observed at optimized condition (208 kPa, natural pH and 200 mg/L concentration). The results obtained in this work indicate that synthesized membrane could be used as proficient microfiltration membrane for protein rejection applications.

References

[1] Kalin, S., Phares, E., Skerrett, P., Dart, H., 2016. The nutrition source: protein. https://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/protein/(accessed 20 march 20018).
[2] Fluegel, S.M., 2013. Dairy bioactives: BSA. https://mooscience.com/Bovine-Serum-Albumin.html (accessed 20 march 20018).
[3] https://rockland-inc.com/bovine-serum-albumin.aspx. (accessed 20 march 20018).
[4] Pereira, M.M., Pedro, S.N., Quental, M.V., Lima, A.S., Coutinho, J.A.P., Freire M.G., 2015. Enhanced extraction of bovine serum albumin with aqueous biphasic systems of phosphonium- and ammonium-based ionic liquids. Journal of Biotechnology, 206, 17–25.
[5] Taha, M., Quentala, M.V., Correia, I., Freire, M.G., Coutinho, J.A.P., 2015. Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good’s buffers ionic liquids. Process Biochemistry, 50, 1158–1166.
[6] Hebbar, H.U., Raghavarao, K.S.M.S., 2007. Extraction of bovine serum albumin using nanoparticulate reverse micelles. Process Biochemistry, 42, 1602–1608.
[7] Chen, L., Dong, J., Guo, X., 2017. Extraction of bovine serum albumin with reverse micelles from glucosylammonium and lactosylammonium surfactants. Process Biochemistry, 60, 108–114.
[8] Pawar, S.S., Regupathi, I., Prasanna, B.D., 2017. Reverse micellar partitioning of Bovine Serum Albumin with novel system. Resource-Efficient Technologies, 3, 491–494.
[9] Pramila, J., Melbiah, J.S.B., Rana, B., Gandhi, N.N., Nagendran, A., Mohan, D., 2018. Permeation characteristics of tailored poly (m-phenylene isophthalamide) ultrafiltration membranes and probing its efficacy on bovine serum albumin separation. Polymer Testing, 67, 218–227.
[10] Saraswathi, M.S.A., Mahalakshmi, S., Vetrivel, S., Divya, K., Rana, D., Nagendran A., 2018. Separation of bovine serum albumin and humic acid contaminants from aqueous stream using tailored poly (amide imide) ultrafiltration membranes. Journal of Environmental Chemical Engineering, 6, 1912–1917.
[11] Gebru, K.B., Das, C., 2017. Removal of bovine serum albumin from wastewater using fouling resistant ultrafiltration membranes based on the blends of cellulose acetate, and PVP-TiO2 nanoparticles. Journal of Environmental Management, 200, 283-294.
[12] Yang, L., Haitao, H., Pengfei, H., Jiyou, G., 2017. Exploration of zwitterionic cellulose acetate antifouling ultrafiltration membrane for bovine serum albumin (BSA) separation. Carbohydrate Polymers. 165, 266-275 (http://dx.doi.org/10.1016/j.carbpol.2017.02.052)
[13] Valiño, V., Román, M.F.S., Ibañez, R., Ortiz, I., 2014. Improved separation of bovine serum albumin and lactoferrin mixtures using charged ultrafiltration membranes. Separation and Purification Technology, 125, 163–169.
[14] Teepakorn, C., Fiaty, K., Charcosset, C., 2015. Optimization of lactoferrin and bovine serum albumin separation using ion-exchange membrane chromatography. Separation and Purification Technology, 151, 292–302.
[15] Fakhfakh, S., Baklouti, S. & Bouaziz, J., 2010. Preparation ,characterization and application in BSA solution of silica ceramic membranes. Desalination, 262, 188-195.
[16] Jedidi, I., Sai, S., Khmakem, S., Elloumi-ammar, N., Fourati, A., Charf, A., 2009. New ceramic microfiltration membranes from mineral coal fly ash. Arabian Journal of Chemistry, 2, 31-39.
[17] Vasanth, D., Pugazhenthi, G., Uppaluri, R., 2013. Cross flow microfiltration of oil-in-water emulsion using low cost ceramic membranes. Desalination, 320, 86-95.
[18] Jana, S., Purkait, M.K., Mohanty, K., 2011. Clay supported polyvinyl acetate coated composite membrane by modified dip coating method: Application for the purification of lysozyme from chicken egg white. Journal of Membrane Science, 382, 243–251.
[19] Tanyolac, D., Sonmezisik, H., Ozdural, A.R., 2005. A low cost porous polyvinylbutyral membrane for BSA adsorption. Biochemical Engineering Journal, 22, 221-228.
[20] Mittal, P., Jana, S., Mohanty, K., 2011. Synthesis of low-cost hydrophilic ceramic-polymeric composite membrane for treatment of oily wastewater. Desalination, 282, 54-62.
[21] Hashino, M., Hirami, K., Ishigami, T., Ohmukai, Y., Maruyama, T., Kubota, N., Matsyama, H., 2011. Journal of Membrane Science, 384, 157-165.
[22] Jedidi, I., Khemakhem S., Saïdi S., Larbot A., Elloumi-Ammar N., Fourati A., Charfi, A., Salaha, A.B., Amar, R.B., 2011. Preparation of a new ceramic microfiltration membrane from mineral coal fly ash: Application to the treatment of the textile dying effluents. Powder Technology, 208, 427–432.
[23] Vasanth, D., Pugazhenthi, G., Uppaluri, G., 2011. Fabrication and properties of low cost ceramic microfiltration membranes for separation of oil and bacteria from its solution. Journal of Membrane Science, 379, 154–163.
[24] Porubská, M., Szöllos, O., Kónová, A., 2012. FTIR spectroscopy study of polyamide-6 irradiated by electron and proton beams. Polymer Degradation and Stability, 97, 523-531.
[25] Sinha, M.K., Purkait, M.K., 2013. Increase in hydrophilicity of polysulfone membrane using polyethylene glycol methyl ether. Journal of Membrane Science, 437, 7-16.
[26] Aravind, K.U., Mathew, J., Aravindkumar, C.T., 2007. Transport studies of BSA, lysozyme and ovalbumin through chitosan/polystyrene sulfone multilayer membrane. Journal of Membrane Science, 299, 146-155.

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Published

2018-09-01

How to Cite

Gupta, V., & J, A. (2018). Protein Separation Using Fly-ash Microfiltration Ceramic Membrane. CSVTU International Journal of Biotechnology, Bioinformatics and Biomedical, 3(2), 17–25. https://doi.org/10.30732/ijbbb.20180302002

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Articles